Four and Two Context and architecture Complexities of the genome Biology Diagrams
BlogFour and Two Context and architecture Complexities of the genome Biology Diagrams During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the
Individual sites display diverse patterns of interphase-to-mitosis dynamics in chromatin accessibility. (A) Example of DNase hotspots and peaks. (B) G1E + GATA1 DNase cut density profiles at the Gata2, Slc8b1, and Klf13 loci are shown to illustrate their spatial patterns. Broad versus narrow sensitivity patterns are captured by the hotspots Although mitotic chromosomes are highly compacted and transcriptionally inert, some active chromatin features are retained during mitosis to ensure the proper postmitotic reestablishment of maternal transcriptional programs, a phenomenon termed "mitotic bookmarking." However, the dynamics and regula โฆ
Chromatin accessibility: biological functions, molecular mechanisms and ... Biology Diagrams
Chromatin accessibility is generally perceived as a common property of active regulatory elements where transcription factors are recruited via DNA-specific interactions and other physico-chemical properties to regulate gene transcription. Recent work in the context of mitosis provides less trivial โฆ The dynamic regulation of chromatin accessibility is one of the prominent characteristics of eukaryotic genome. The inaccessible regions are mainly located in heterochromatin, which is multilevel compressed and access restricted. HNF4a and C/EBPฮฑ, as well as the cell cycle and mitosis repressive factor, E2F4, to the their target genes
Lastly, mitotic chromosomes have also been investigated as a natural perturbation of chromatin structure. In mitosis, transcription is globally downregulated, Thus, chromatin accessibility can play an important role in the sensing lesion depending on the type of DNA damage, and more work in this direction will shed mechanistic light on the
A changing paradigm of transcriptional memory propagation through mitosis Biology Diagrams
Despite chromosome condensation during mitosis visible by microscopy, the landscape of chromatin accessibility at the macromolecular level is largely unaltered. However, mitotic chromatin accessibility is locally dynamic, with individual loci maintaining none, some, or all of their interphase accessibility.
These studies clearly demonstrate that the pronounced compaction of chromatin during the transition from interphase to mitosis 1 does not markedly affect its local accessibility and thus, possibly
